Rapa-induced apoptosis is blocked by FK506.
(A) Immature mo-DCs were cultured in RPMI–10% FCS supplemented with IL-4 and GM-CSF in the absence or presence of 10−7 M Rapa for 48 hours. Before addition of Rapa, the cells were preincubated with CsA or FK506 (10−6 M). Apoptosis was detected by flow cytometry using annexin V/PI staining. The percentage of apoptosis demonstrates the percentage of annexin V+ PI−cells. Data shown are the mean ± SD of duplicate cultures. The results are representative of 3 independent experiments. (B) Immature mo-DCs were cultured in RPMI–10% FCS supplemented with IL-4 and GM-CSF in the absence or presence of 10−7 M Rapa for 48 hours. FK506 (5 × 10−6 M) was added to the cultures at the indicated time points. Apoptosis was detected by flow cytometry using annexin V/PI staining. The percentage of apoptosis demonstrates the percentage of annexin V+ PI− cells. Data shown are the mean ± SD of duplicate cultures. The results are representative of 3 independent experiments.
