Surface phenotype of spleen DCs, B lymphocytes, and macrophages by rare event flow cytometry.

Expression of surface molecules on gated DC (R1*R4), B lymphocytes (R2), and macrophages (R3). Gates were defined as follows: R1, Cocktail⁻ HLA-DR⁺ events; R2, Cocktail⁺ HLA⁻ DR⁺ events; R3, Cocktail⁺⁺ events. The cocktail consisted of anti-CD3, CD14, CD16, and CD19 PE-conjugated antibodies as described in “Materials and methods.” (A-C) Data from donor 103. (D) Preferential expression of CD11c on Cocktail⁻HLA-DR⁺ DC. Data from donor 94.