Distribution of CD11c⁺ spleen DC in normal donors.

(A,B) Triple staining for nuclei (Hoechst, A) CD11c (Texas Red, B), and CD14 (FITC, B). Donor 147; original magnification, ×200. Strongly labeled CD11c⁺ DCs are scattered throughout the white pulp (WP) and surround its periphery, just inside red pulp (RP) CD14⁺ macrophages. (C,D) Triple staining for nuclei (Hoechst, C), CD11c (Texas Red, D), and CD3 (FITC, D). Donor 108; original magnification, ×200. Strongly labeled CD11c⁺ DCs are found mostly in the T-cell zone (T) and the marginal zone. (E,F) Triple staining for nuclei (Hoechst, E), CD11c (Texas Red, F), and CD19⁺CD20 (FITC, F). Donor 173; original magnification, ×200. CD11c⁺ CD19⁻CD20⁻ cells are found around the edge and in the interior of the B-cell zone (B). (G,H) Triple staining for nuclei (Hoechst, G), CD86 (Texas Red, H), and CD83 (FITC, H) in a section of white pulp. Donor 147; original magnification, ×400. Rare CD86⁺ or CD83⁺ cells are found in the white pulp. Distribution and phenotype of spleen DCs observed in these donors were representative of those found in 5 organ donors.