SDF-1α induces the tyrosine phosphorylation of JAK family members.

Total cell lysates from untreated or SDF-1α-treated (20 nM) CTS cells were immunoprecipitated (IP) with anti-JAK1, anti-JAK2, anti-JAK3, or anti-TYK2 antibodies and analyzed by Western blotting (WB) with PY99 antiphosphotyrosine antibody (pTyr) (A-D, upper panels). The protein loading was verified by reprobing the membranes with the same antibodies used for the immunoprecipitations (A-D, lower panels).