Fig. 4.
Association of JAK2 with PI3-K (p85 subunit).
CTS cells were pretreated with AG490 (+) or DMSO (−) for 2 hours and then stimulated with SDF-1α (20 nM) for 1 minute. Total cell lysates were immunoprecipitated with anti-JAK2 antibody and analyzed by Western blotting with anti-p85 antibody.