Amino acid sequence, homology, and Northern blot analysis.

(A) Amino acid sequence of EAF1. The underlined sequence is displayed in panel B. (B) Homology of EAF1 to LAF4 and AF4. The region of homology identified from the DeCypher II search is rich in serine, aspartic acid, and glutamic acid residues. Identical residues are indicated in black boxes and conservative residues by gray boxes. (C) Northern blot analysis. Human multiple tissues were hybridized with anEAF1 cDNA probe. EAF1 is broadly expressed in multiple tissues except in thymus. Splice variants are seen in skeletal muscle and testis. The same blot was probed with human β-actin as a control for RNA loading.