EAF1 contains a transactivation domain that localizes to its C-terminus that is homologous to the LAF4 and AF4 transactivation domains.

(A) Schematic diagram of the GAL4-EAF1, AF4, and LAF4 constructs used for the CAT-ELISA assay. (B) Western blot of protein extracts from 293 cells transfected with GAL4 fusions. (C) Transcriptional activation assay. The indicated fold activation levels are relative to those of the controls. Each bar represents the mean ± SD of at least 3 independent experiments.