ELL colocalizes with EAF1 in nuclear speckles.

(A) DAPI staining of HeLa cells. (B) Immunofluorescence with the affinity-purified polyclonal ELL antibody detected with fluorescein isothiocyanate (FITC)-labeled goat antirabbit antibodies. (C) Dual fluorescence imaging of ELL and DAPI showing that the speckles are nuclear. (D) DAPI staining of HeLa cells. (E) Immunofluorescence with the monoclonal EAF1 antibody detected with RRX-labeled goat antimouse antibodies. (F) Dual fluorescence imaging of EAF1 and DAPI. (G) Confocal microscopy with the affinity-purified polyclonal ELL antibody detected with FITC-labeled goat antirabbit antibodies. (H) Confocal microscopy with the monoclonal EAF1 antibody detected with Cy5-labeled goat antimouse antibodies. (I) Merged confocal image of ELL and EAF1 revealed colocalization.