Delocalization of EAF1 by transient expression of MLL-ELL.

HeLa cells were transiently transfected with FLAG-tagged ELL, MLL-ELL, and the amino-terminus of MLL. Expression of these constructs was visualized by confocal microscopy using a biotinylated anti-FLAG antibody and streptavidin-FITC, and expression of endogenous EAF1 was visualized using the EAF1 monoclonal and Cy-5 labeled goat antimouse antibodies. In the upper row, an overall increase in the number of EAF1 speckles was observed in the cells transfected with FLAG-ELL. In the middle row, EAF1 was delocalized from its typical speckled distribution by expression of FLAG-MLL-ELL. In the lower row, the EAF1 pattern was not affected by expression of the amino-terminus of MLL.