Up-regulation of Bcl-xL and c-myc in MO7e cells transduced with D816H mutant c-kit.
MO7e cells transduced with empty pLNCX vector or wild-type c-kit were SCF-starved for 5 hours, and then stimulated, together with the cells transduced with D816H mutant c-kit, with rhSCF (40 ng/mL) for the times indicated. (A) Western blot (WB) analysis of the total cellular extracts from the cells. The blot in the upper panel was probed with anti–Bcl-xL antibody. The blot was stripped and reprobed with antitubulin antibody to verify the protein loading, as shown in the lower panel. (B) Northern blot (NB) analysis of total RNA from the indicated cell types. The blot in the upper panel was hybridized with a c-myc–specific probe as described in “Materials and methods.” The blot was stripped and rehybridized with a GAPDH-specific probe to verify RNA loading, as shown in the lower panel.
