Outcome of plasmid cDNA-based antigen loading of 34-LCs.

IFN-γ production by the CTL clone was measured after coculture with HLA-A2⁺ 34-LCs electroporated with various plasmid DNA constructs encoding Melan-A (pcDNA1.1/Melan-A; n = 12), EGFP (pEGFP-N1; n = 12), luciferase (pCMV-Luc; n = 3), or with a backbone vector (pcDNA1.1/Amp; n = 6) lacking a eukaryotic cDNA sequence. Alternatively, 34-LCs were electroporated with in vitro–transcribed mRNA encoding EGFP or Melan-A (n = 3). Results are shown as mean ± SD. * P < .05.