Strategy and results of α-thalassemia multiplex polymerase chain reaction analysis.

(A) Schematic representation of the α-globin gene cluster, indicating extents of the 7 deletions and relative positions of the primers (except for the control LIS1-F and LIS1-R primers, which are located on a different chromosome). Locations of X, Y, and Z sequence homology boxes and hypervariable regions (HVRs) are also shown. (B) Multiplex PCR results from genomic DNA samples with various α-globin genotypes. M indicates Generuler 1kb DNA ladder (Fermentas, St Leon-Rot, Germany).