Fig. 4.
Cytokine-induced activation is inhibited by cytochalasin D.
Cytokine-starved Ba/F3_FcαRI cells (A) or purified eosinophils (B) were incubated with buffer (■) or with cytochalasin D for 10 minutes and subsequently stimulated or left untreated. Ba/F3_FcαRI cells were incubated with IL-3 (1:1000; 15 minutes) (A) and eosinophils were stimulated with IL-5 (10−9M; 15 minutes) (B). Binding of IgA beads to these cells was measured and results are expressed as rosette index (number of beads/100 cells) and as means ± SE (n = 3).
