Chemically synthesized ONOO⁻ induces human thymocyte apoptosis.

Flow cytometric analysis of annexin V staining after 4 and 18 hours of exposure to various concentrations of ONOO⁻. The percentage of annexin⁺ cells was time- and concentration-dependent, reaching maximum values (> 80%) at 18 hours with 500 μM ONOO. The analysis was performed on the whole thymocyte population. The data are representative of 3 individual experiments.