Fig. 5.
Exogenous SOD inhibits the apoptotic effect of SIN-1.
Cells were treated with 0.5 nM SIN-1 and various concentrations of SOD for 4 hours. SIN-1 induced annexin V staining of 31% of cells after 4 hours. Addition of SOD strikingly reduced the percentage of annexin V+ cells in a concentration-dependent manner. The analysis was performed on the whole thymocyte population. The data are representative of 3 individual experiments.