![Fig. 2. Bone marrow reconstitution using a retrovirus. / Bone marrow from female recipient mice was reconstituted with genetically engineered male donor cells using a transplantation-based method. Eight weeks later, DNA was extracted from the bone marrow, spleen, and liver of these mice and subjected to PCR for mouse Y antigen (395 base pairs [bp]). A representative photograph from 9 chimeric mice is shown (A). DNA from spleen was also subjected to semiquantitative PCR for the human IL-1Ra gene. This pair of primers may yield both cDNA (263 bp) and gDNA (1045 bp).24 As a standard, different amounts of IL-1Ra cDNA, corresponding to 1, 0.5, 0.2, 0.1, and 0.05 copies per cell, were used. A representative photograph from 4 individual mice is shown. M: DNA marker (φ × 174/HaeIII digest) (B).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/1/10.1182_blood.v98.1.57/6/h81311232002.jpeg?Expires=1735515920&Signature=1aTVOBbcExR0gyytXK2WvXgFinEiST6pYjbnXJswZhOA64VXnGTrxePXwqrh654GKF6hftZV4tBTQ1JCvqZy8q8q3-a1ZuhrTYr8FIoAXKlgnuOpYknOe0XQBa7~nuWcpFPbnE01K5XDBp5WaiOSCuKkoHFmXFubt79erLZTq08XmX1a16gcxtO-8s319RHppc7JrTeIrcLa3YBaHwP9XVoQx3b1ikz3zz~-HfLuK3XIwo0j0jLhLxgkMHefyfFxVdmSMVKzfkTjJzltxWkvPYkbnwlDvsxRnLBpRmCAh1si4XPWkDEIizlELbcWhWSShY4~Zi38TKswbsb9ErTsrA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Bone marrow reconstitution using a retrovirus.
Bone marrow from female recipient mice was reconstituted with genetically engineered male donor cells using a transplantation-based method. Eight weeks later, DNA was extracted from the bone marrow, spleen, and liver of these mice and subjected to PCR for mouse Y antigen (395 base pairs [bp]). A representative photograph from 9 chimeric mice is shown (A). DNA from spleen was also subjected to semiquantitative PCR for the human IL-1Ra gene. This pair of primers may yield both cDNA (263 bp) and gDNA (1045 bp).24 As a standard, different amounts of IL-1Ra cDNA, corresponding to 1, 0.5, 0.2, 0.1, and 0.05 copies per cell, were used. A representative photograph from 4 individual mice is shown. M: DNA marker (φ × 174/HaeIII digest) (B).
