Platelet glycoprotein expression analysis in PRP.

(A) Flow cytometry of a platelet nonspecific protein (GST) and GPIbα in the platelets of a control (Crl) or patient V:3. The platelets of V:3 are at least 2 to 3 times larger than control platelets, and a subgroup seems immature with very low levels of GPIbα (indicated by an arrow). (B) Normalized (versus GPIIIa expression) FACS results for the expression of GST, GPIbα, and GPIbβ in control (Crl), obligate carriers (VI:5 and IV:1), and patient V:3. ░, GST/GPIIIa; ▪, GPIbα/GPIIIa (upper panel) and GPIbβ/GPIIIa (lower panel). (C) Platelet glycoprotein expression using Western blot analysis to study the expression of GPIIIa, GPIbα, and GPIbβ. The expression of the GATA1-independent large Gsα subunit (52 kd) was also studied.