Comparison of the expression level between human and baboon CD34⁺ cells for genes selected from different abundance categories, by semiquantitative RT-PCR.

Five known genes representative of each of the abundance categories described in Figure 2 were analyzed by RT-PCR using primers from the 3′-untranslated region of the gene. The PCR reactions were done with (+) or without (−) addition of reverse transcriptase (RT) for the indicated cycle number (Cy). The genes tested are: TM4SF4, transmembrane 4 superfamily member 4; PTK9, protein tyrosine kinase 9; CYP1B1, cytochrome P450, subfamily 1 (dioxine-inducible), polypeptide 1 (glaucoma 3, primary infantile);CSF3R, colony-stimulating factor 3 receptor; B2M, β₂-microglobulin. The intensity measured with GeneFilters is compared to that measured by RT-PCR.