The disseminated lymphoma seen in MCL1 transgenic mice exhibits clonal rearrangement of the immunoglobulin heavy-chain locus.
A probe representing the murine immunoglobulin heavy-chain JH region probe was used in Southern blotting of cases of follicular lymphoma (lanes 3-4), diffuse large-cell lymphoma (lanes 5-7, 9, 11-14), and polymorphic lymphoma (lanes 2, 8, 10). Tissues (LN, lymph node; Sp, spleen; Li, liver) from the same animal are indicated with horizontal overlining. In the animal represented in lanes 5-7, lymphoma was present in the lymph nodes, spleen, and liver. In the animal represented in lanes 11-14, lymphoma was present in the lymph nodes and spleen but had not massively infiltrated the liver. The position of the germline (unrearranged) band is indicated with a G. Tr indicates transgenic.
