CD28 stimulation leads to up-regulation of the IL-8 promoter in myeloma cells.

RPMI 8226 myeloma cells were transfected as in Figure 3, with either a wild-type IL-8 promoter or ones containing mutations at either the NF-κB site or AP-1 site within the IL-8 RE/AP sites. Cells were stimulated for 18 hours. Raw luciferase units were used without standardization. The results shown are the average of 3 independent transfections. Error bars reflect the SD.