Proliferation and differentiation potentials for CD34⁺CD81⁺, CD34^lowCD81⁺, and CD34⁺CD81^high cells in suspension cultures.

(A) Proliferation of 1 × 10³CD34⁺CD81⁺ (○), CD 34^lowCD81⁺ (▪), and CD34⁺CD81^high cells (▵) in a time-course experiment. (B) Photographs of progenies derived from CD34⁺CD81⁺ and CD34^lowCD81⁺ cells in suspension cultures on day 12. A tendency for CD34⁺CD81⁺ cells to generate myelocytes and for CD34^lowCD81⁺ cells to generate erythrocytes is shown (× 400). (C) Low cytometric profiles of expression of CD81 and CD34 on progenies derived from CD34⁺CD81⁺ and CD34^lowCD81⁺ cells in suspension cultures on day 14. CD34⁺ cells were obtained only from the CD34⁺CD81⁺ fraction.