Antigen-specific CD8 T cells from healthy donors and HIV-seropositive donors (various disease stages), stained with A0201 and B8 tetramers for HIV, CMV, and EBV epitopes, do not express LN-homing receptors.

Representative dot plots for costaining of the indicated tetramers with CCR7, CD62L, and CD45RA are shown. Although the pattern of CCR7 and CD62L staining for tetramer⁺ CD8 T cells responding to all 3 viruses was similar, CD45RA staining was reduced in HIV-specific T cells compared with cells specific for the other viruses causing chronic infections.