Expression of cyclin D3 in MM cell lines.

(A) Northern blot containing 30 μg (10 μg for sample marked by asterisk) total RNA from each MM cell line was hybridized with a cyclin D3 probe. A portion of the film encompassing the 2.4-kb cyclin D3 mRNA is shown for 15- and 96-hour exposures. Ethidium bromide–stained 2.0-kb ribosomal RNA (rRNA) is shown as a loading control. (B) Western blot containing 100 μg total protein for each of 2 MM cell lines was probed with an anti–cyclin D3 antibody (no. SC-182). A portion of the film containing the 33-kd cyclin D3 band is shown.