Three-color FISH analysis of the KMM-1 MM line.

(A) CH (green), cyclin D3 cosmid (red), and chromosome 6 painting (purple) probes were hybridized to metaphase chromosomes (blue DAPI counterstain). Two copies of CH and cyclin D3 colocalize (arrowheads) on der(14). Two copies of CH (arrows) colocalize with VH and inserted c-myc (not shown). One copy of cyclin D3 is on normal chromosome 6, and 3 additional copies of cyclin D3 are on rearranged chromosome 6. (B) Interphase nucleus from (A) shows colocalization of 2 sets of CH and cyclin D3 probes (arrowheads).