Generation of IL-1α, IL-1β, TNF-α, and TF by plasmin-stimulated human monocytes.

Monocytes were incubated with various concentrations of plasmin for the times indicated. Monocytes treated with LPS 1 μg/mL served as positive controls. Supernatants were collected, and the release of IL-1α, IL-1β, and TNF-α was analyzed by ELISA. Cell-associated TF was measured in whole-cell lysates. Results are the mean ± SEM of 4 to 6 independent experiments. *P < .05 and **P < .01. versus unstimulated controls.