AP-1 activation in plasmin-stimulated monocytes.

Binding to the AP-1 site was analyzed using EMSA. (A) Nuclear extracts (10 μg) from monocytes either unstimulated or stimulated for 2 hours with plasmin 0.43 CTA U/mL were incubated with the³²P-labeled DNA probe containing the AP-1 binding site. In competition experiments the effects of specific (AP-1) and nonspecific (SP1) competitors at 100-fold molar excess were analyzed. (B) Composition of the AP-1 complexes was characterized by supershift analysis with antibodies against Jun and Fos proteins. Results of 1 of 3 experiments are shown.