Effect of IDV on cell-cycle progression.

Cell-cycle progression of Jurkat (A) and PM1 T (B) cells and apoptosis in Jurkat (C) and PM1 (D) T-cell lines. For cell-cycle analysis Jurkat and PM1 T cells were cultured with different concentrations of IDV for 24 (⋄), 48 (♦), and 72 (×) hours. Cell-cycle progression was determined by propidium iodide staining and is represented as the percentage of cells in S + G2/M phase by using multicycle flow cytometry software. To study the effect of IDV on anti-Fas–induced apoptosis in Jurkat and PM1 T cells, cells were cultured without or with anti-Fas mAb CH11 (100 ng/mL) in presence of different concentrations of IDV, and apoptosis was determined at 2 (▵), 6 (■), and 18 (●) hours. Cells were also precultured with different concentrations of IDV for 18 hours, and apoptosis was induced by culturing these cells with CH11 for 6 (▪) and 18 (●) hours. Data are shown for mean ± SD of the percentage of cells undergoing apoptosis as estimated by propidium iodide staining.