Effect of IDV on caspases-1, -3, -4, -5, -8, and -9.

Cell lysates from apoptotic Jurkat T cells were preincubated with different concentrations of IDV (▿, 12.5 μM; ■, 25 μM; ▪, 50 μM; and ♦, 100 μM) or with 100 μM of specific caspase inhibitors (▾) followed by assay for caspase activity as described in the “Materials and methods” section by the release of 7-amino-4-trifluoromethyl coumarin, expressed as relative fluorescence units (RFU), from caspase-specific fluorogenic substrates over a 2-hour period. Controls consisted of wells without lysates (●) and of lysates without inhibitors (○).