Southern blot analysis of DNA from leukemia cells with t(5;10)(q33;q22).

Genomic DNA was isolated mononuclear cells from Ficoll-Hypaque density gradient sedimentation of peripheral blood from the patient and a normal donor and was digested (10 μg) with 5 different restriction endonucleases. The Southern blot was probed with a 1.1-kb (XhoI/HindIII) genomic PDGFβR probe. Rearrangements were evident in patient DNA when the restriction endonucleases HindIII, PstI, or PvuI were used (lanes 3-8).