Analysis of proviral integration in disease tissue in transplanted mice.

Southern blot analysis was performed with 10 μg DNA extracted from murine tissues. (A) DNA was digested with the restriction enzymeNheI, which cleaves in the viral long-terminal repeat sequences and demonstrates viral integration. The expected DNA band size is 5.8 kb. (B) Clonality of the disease was assessed by digestion with EcoRI, which cleaves once within the provirus. There is clonal disease in spleen and bone marrow, and the presence of a second clone was detected in lymph node and thymus. (C) Restriction sites within the proviral sequence.