HSV amplicon vectors are capable of infecting CLL cells and expressing B7.1.

CLL cells were isolated from human subjects and were left untreated (A) or were transduced with equivalent transduction units of HSVlac (B), hf-HSVlac (C), HSVB7.1 (D), or hf-HSVB7.1 (E). Cell surface expression of B7.1 was assessed by fluorescence-activated cell sorting; representative flow diagrams for each treatment are shown. The y-axis illustrates forward scatter increasing from bottom to top, and the x-axis illustrates positive B7.1 staining increasing in intensity from left to right. Comparable results were obtained from 8 different patients.