A specific autologous CTL response can be generated against CLL cells transduced with hf-HSVCD40L stocks.

T cells purified from the PBMCs of a donor with CLL were incubated with control (♦), hf-HSVlac–infected (▪), or hf-HSVCD40L–infected (▴) autologous CLL cells. Cytotoxicity assays were performed by incubating these primed T cells with ⁵¹Cr-labeled autologous CLL cells in the absence (A) or the presence (B) of anti-MHC monoclonal antibody, W-6.32, or with ⁵¹Cr-labeled allogeneic CLL cells (C) at varying effector-target (E/T) ratios. Supernatant was collected, and radioactivity was measured using a γ counter. Mean values were calculated for the triplicate wells, and the results are expressed as percentage specific lysis according to the following formula: experimental counts − spontaneous counts/total counts − spontaneous counts × 100. Error bars represent the standard deviation of triplicate wells. Data are representative of 1 of 2 experiments.