Fig. 1.
Fig. 1. Targeted disruption of Cd59. / (A) A gene-targeting vector was constructed in which exon 3, the major coding portion of the protein, was replaced by the neomycin-resistance gene (neo). The herpes simplex virus thymidine kinase cassette (HSV-tk) was inserted at the 3′ end of the homologous region (delineated by dotted lines). Double-headed arrows indicate the size of fragments seen in Southern blot analysis of wild-type (top) and targeted (bottom) alleles afterBglII digestion of genomic DNA hybridized to the external probe (hatched box). (B) Southern blot analysis of ES cell DNA showing wild-type and targeted clones. (C) PCR genotyping of genomic DNA derived from the progeny of CD59+/− mice showing the 200-bp fragment amplified from the wild-type allele (with primers P1 and P3) and the 450-bp fragment amplified from the targeted allele (with primers P2 and P3).

Targeted disruption of Cd59.

(A) A gene-targeting vector was constructed in which exon 3, the major coding portion of the protein, was replaced by the neomycin-resistance gene (neo). The herpes simplex virus thymidine kinase cassette (HSV-tk) was inserted at the 3′ end of the homologous region (delineated by dotted lines). Double-headed arrows indicate the size of fragments seen in Southern blot analysis of wild-type (top) and targeted (bottom) alleles afterBglII digestion of genomic DNA hybridized to the external probe (hatched box). (B) Southern blot analysis of ES cell DNA showing wild-type and targeted clones. (C) PCR genotyping of genomic DNA derived from the progeny of CD59+/− mice showing the 200-bp fragment amplified from the wild-type allele (with primers P1 and P3) and the 450-bp fragment amplified from the targeted allele (with primers P2 and P3).

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