CD59 expression on erythrocytes of CD59^+/+, CD59^+/−, and CD59^−/− mice.

Erythrocytes were isolated by centrifugation, labeled with a biotinylated anti-CD59 mAb followed by phycoerythrin-conjugated streptavidin, and gated on the forward scatter versus side scatter dot plot generated by the FACSCalibur. (A) Overlay of the histogram profiles of CD59^+/+ (unshaded), CD59^+/−(lightly shaded), and CD59^−/− (heavily shaded) erythrocytes. (B) Graph showing the CD59 mean cell fluorescence (MCF) obtained for erythrocytes from CD59^+/+, CD59^+/−, and CD59^−/− mice (filled bars; 3 in each group). The negative controls shown underwent the same procedure but without the anti-CD59 mAb being present (open bars). Error bars represent SD for the 3 determinations.