Intravascular hemolysis and hemoglobinuria in CD59^−/− mice.

(A) CD59^+/+, CD59^+/−, and CD59^−/− mice (6 CD59^−/−, 5 in other groups) were bled into EDTA tubes and the plasma was promptly separated. The hemoglobin concentration (cHb) in each plasma sample was measured by spectrophotometry of the diluted plasma sample at 3 wavelengths and application of a published formula. Male mice are represented by squares, females by triangles. Of note, the 3 mice in the CD59^−/− group with the highest cHb were all male. Similar results were obtained with a second set of mice. (B) Urine was collected from CD59^+/+, CD59^+/−, and CD59^−/− mice (6 CD59^−/−, 5 in other groups), and absorbance at 412 nm in the undiluted urine was measured. Male mice are represented by squares, females by triangles.