CVF induces further intravascular hemolysis in CD59^−/− mice.

Mice were bled prior to intraperitoneal injection of CVF. After 1 hour the mice were exsanguinated. Plasma was separated from the pre-CVF and post-CVF bleeds, and cHb was measured by spectrophotometry as described in the “Materials and methods” section. Male mice are depicted by squares, females by triangles. (A) In CD59^+/+ mice, cHb was low in the pre-CVF sample and, although cHb increased in all mice (lines join pre- and postsamples for individual mice), remained low post-CVF. (B) In CD59^−/− mice, cHb was high in the pre-CVF samples and, in some mice, increased to very high levels after CVF treatment. Of note, the 3 mice with the highest pre- and post-CVF cHb levels were male; the other 3, showing modest increases, were female. Note different scales in (A) and (B).