Immunoblot of the plasmic digest of normal fibrinogen and fibrinogen Milano XII obtained in the presence of 5 mM Ca⁺⁺ or 5 mM EDTA.

Detection antibodies: polyclonal antifibrinogen antibody (A), polyclonal antibody to fibrinogen γ-chain (B), monoclonal antibody to β 15-21(C), monoclonal antibody to human serum albumin (D). Fibrinogen (1.5 mg/mL) was digested with plasminogen (10 μg/mL) and streptokinase (40 U/mL) for 4 hours at 37°C. Amount of fibrinogen loaded: 0.5 μg (A); 1 μg (B); 15 μg (C); 30 μg (D). * indicates bands that resulted from a slight albumin contamination of the purified fibrinogens; N, normal fibrinogen; M, fibrinogen Milano XII.