Immunophenotype of CD34⁺-enriched cord blood cells.

(A,B) Isotype controls on CD34⁺ column-enriched cells from lymphoid region defined by forward and side scatter (R1 not shown). (C) CD34⁺ column-enriched cells gated on R1. Shown are R2 gate (CD34⁺CD38⁻ cells) and R3 gate (CD34⁺ cells). (D) CD38 and CD7 expression on CD34⁺ cells gated from R3. (E) CD34⁺CD38⁻ cells gated from R2. Shown is the CD7⁺ sort gate (R4). (F) Re-analysis of CD34⁺CD38⁻CD7⁺ cells isolated from R4 (98.9% purity).