Sorting strategy for AML cells.

(A) Control staining of AML cells with Hoechst 33342 and verapamil. (B) The FACS profile of AML cells stained with Hoechst 33342 alone, with gate R2 defined as SP⁺ cells. (C) FACS profile of AML cells stained with CD34-FITC and CD38-PE. Gates were set using the verapamil and IgG FITC/PE controls. The following subpopulations were sorted: R4 and R5 within R2 and R4 within R3.