UV light–induced DNA damage, apoptosis, and cytochrome

c release. (A) UV light–induced DNA damage. DNA double-strand breaks were assessed by alkaline unwinding assay. (B) UV light–induced apoptosis was assessed by flow cytometry. (C) Detection of cytochrome c release by Western blotting. 50 μg cytosolic protein or 25 μg mitochondrial protein was analyzed by immunoblotting with denatured anticytochrome c antibody (7H8.2C12) at 1:1000 dilution.