Fig. 2.
Fig. 2. Autoradiographs depicting donor leukocyte survival in 3 HIV-infected female recipients (NLR-1, NLR-2, and NLR-4) (top 3 rows). / Amplification of Y-chromosome DNA was performed using duplicate 125 μL whole blood samples collected at weekly intervals. All T00 (pretransfusion) samples were negative. The T07 blood sample from NLR-1 during the second study transfusion was positive (though a weak band was seen at T14, quantitation was below the definition of positive). NLR-2 was positive on T07, during the second study transfusion. NLR-4 was strongly positive on T14, during the first study transfusion. Lysate standards run with the clinical samples (NLR-1, standard run 2; NLR-2 and NLR-4, standard run 1) demonstrate 10-fold dilutions starting from the leftmost band equivalent to values of 1000, 100, 10, 1 and 0.1 genome equivalents (gEq)/lane.

Autoradiographs depicting donor leukocyte survival in 3 HIV-infected female recipients (NLR-1, NLR-2, and NLR-4) (top 3 rows).

Amplification of Y-chromosome DNA was performed using duplicate 125 μL whole blood samples collected at weekly intervals. All T00 (pretransfusion) samples were negative. The T07 blood sample from NLR-1 during the second study transfusion was positive (though a weak band was seen at T14, quantitation was below the definition of positive). NLR-2 was positive on T07, during the second study transfusion. NLR-4 was strongly positive on T14, during the first study transfusion. Lysate standards run with the clinical samples (NLR-1, standard run 2; NLR-2 and NLR-4, standard run 1) demonstrate 10-fold dilutions starting from the leftmost band equivalent to values of 1000, 100, 10, 1 and 0.1 genome equivalents (gEq)/lane.

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