Immunoblotting of exocytosed material.

After stimulation, the neutrophils were pelleted and the supernatant containing the exocytosed material was analyzed by SDS-PAGE and immunoblotting with anti–hCAP-18 antibodies. (A) Exocytosed material from neutrophils harvested from skin windows (10⁷cells/mL), unstimulated cells (lane a) or cells stimulated with fMLP (lane b), IgG-coated latex beads (lane c), or ionomycin (lane d). (B) Exocytosed material from neutrophils isolated from peripheral blood (10⁷ cells/mL), unstimulated cells (lane a) or cells stimulated with fMLP (lane b), IgG-coated latex beads (lane c), or ionomycin (lane d). (C) Exocytosed material from fMLP-stimulated neutrophils (3 × 10⁸ cells/mL) from peripheral blood.