Immunoblotting of hCAP-18 after incubation with azurophil granule proteins.

Purified hCAP-18 was incubated with azurophil granule proteins. Samples were run on SDS-PAGE followed by immunoblotting with anti–hCAP-18 antibodies. Purified hCAP-18 (lane a) incubated with azurophil granule proteins (lanes b-d) and phenylmethylsulfonyl fluoride (lane c) or aprotinin (lane d).