Immunoblotting with monoclonal anti–LL-37 antibody.

All samples were run on SDS-PAGE followed by immunoblotting with monoclonal anti–LL-37 antibody. Lane a, purified hCAP-18; lane b, exocytosed material from ionomycin-stimulated neutrophils; lane c, purified hCAP-18 incubated with azurophil granule proteins; lane d, elastase; lane e, cathepsin G; lane f, proteinase 3; lane g, with exocytosed material from ionomycin-stimulated neutrophils after depletion of the endogenous hCAP-18.