Cleavage experiment with exocytosed material.

Exocytosed material from ionomycin-stimulated neutrophils was depleted of endogenous hCAP-18 and subsequently incubated with purified hCAP-18 with or without protease inhibitors. Samples were run on SDS-PAGE followed by immunoblotting with anti–hCAP-18 antibodies. Purified hCAP-18 (lane a) incubated with exocytosed material (lanes b-e). This resulted in cleavage of hCAP-18 (lane b). This cleavage was inhibited by the addition of CMK (lane c) but not by chymostatin (lane d) or SLPI (lane e).