Differential inhibition of hCAP-18 cleavage by serine proteases from azurophil granules.

Samples were run on SDS-PAGE followed by immunoblotting with anti–hCAP-18 antibodies. (A) hCAP-18 (lane a) was incubated with elastase alone (lane b) or together with the inhibitors CMK (lane c), chymostatin (lane d), or SLPI (lane e). (B) hCAP-18 (lane a) was incubated with cathepsin G alone (lane b) or together with the inhibitors CMK (lane c), chymostatin (lane d,) or SLPI (lane e). (C) hCAP-18 (lane a) was incubated with proteinase 3 alone (lane b) or together with the inhibitors CMK (lane c), chymostatin (lane d), or SLPI (lane e).