VEGF-mediated stimulation of endothelial cell migration by NB4 CM.

(A) Conditioned media from NB4 cells strongly stimulated endothelial cell migration. Migration was completely inhibited by the addition of antibodies to VEGF. The addition of anti-bFGF antibodies had no effect on endothelial cell migration. The upper horizontal line is a positive control using purified VEGF. The lower horizontal line is a negative control using bovine serum albumin. (B) In contrast to CM from untreated NB4 cells, CM from NB4 cells treated with ATRA did not stimulate endothelial cell migration. The addition of CM from NB4 cells treated with ATRA to CM from untreated NB4 cells did not result in the inhibition of endothelial cell migration.