Fig. 5.
Immunoblots of HIF-1α protein in normoxic and hypoxic Hep3B cells under different conditions.
(A) Untreated Hep3B cells (lanes 1 and 2), Hep3B cells after 3 weeks' culture in medium containing ethidium bromide (EB) (50 ng/mL) to deplete mtDNA to ρ0 status (lanes 5 and 6), or for 3 weeks in the presence of ethidium bromide (50 ng/mL) followed by 1 week in the presence of rotenone (ROT) (1 μg/mL) (lanes 3 and 4). (B) Wild-type Hep3B cells grown under normal conditions or in the presence of rotenone (1 μg/mL) for 1 week. Cells were cultured in parallel for 4 hours in normoxia (21% O2, N) or hypoxia (0.1% O2, H).
