Fig. 4.
Quantitation of Dlk mRNA in the Blast Bank samples from patients with MDS, AML, or CML.
The cDNA prepared from the blasts of 22 patients with MDS, 31 with AML, and 8 with CML was subjected to real-time PCR with primers specific for Dlk or β-actin. The ratio of the abundance of Dlk transcripts to that of β-actin transcripts (Dlk/β-actin) was calculated as 2n, where n is theCT value of β-actin minus theCT value of Dlk, and was normalized by the value of the sample from healthy volunteers (N). C indicates CMMoL; RB, RAEB; TLD, AML with trilineage dysplasia; CP, chronic phase; AP, accelerated phase; and BC, blast crisis. The numbers 0 to 7 refer to the AML subtypes M0 to M7. The letters a, b, and c refer to patients discussed in the text.
