![Fig. 6. PAI-1 in control and QPD platelets. / (A) PAI-1 in control (C) and QPD (Q; 3 patients [Pt] are shown) samples was analyzed by Western blotting with rabbit anti–human PAI-1 after 10% nonreduced SDS-PAGE. Lanes compare 5 μL lysate (L) and ionophore releasates (R), 20 μL K562 media, and 5 μL control ionophore releasate, incubated with 0 to 400 ng/mL tcu-PA (CR + tcuPA), as indicated. Lane * shows the PAI-1 affinity purified from QL using monoclonal anti–u-PA. QPD platelet lysates contained PAI-1 in high–molecular weight complexes (bands near the 98-kd marker) that comigrated with the complexes generated by adding tcu-PA to control releasates. Proteolyzed PAI-1 (arrow) was detected in QPD platelet lysates and in long exposures (not shown) of control releasates incubated with tcu-PA. (B) u-PA–PAI-1 complex ELISA indicated that, unlike control samples, QPD platelet lysates and releasates were unable to generate additional u-PA–PAI-1 complexes in vitro with added (+) tcu-PA (data representative of 2 separate experiments with pooled samples).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/2/10.1182_blood.v98.2.257/5/h81411272006.jpeg?Expires=1735827107&Signature=Dm~rlnXqgBg4X86k~554xWVyk~k1Ysi49LRp4~4G03~CDSqxf8AYU~yA0sG7WHd62jgmLRMWnfjouS6Em~zjjt~Co4hSy1-S4JYinQRG5eXFwB~QAL6zV0knIlEX-AbOic57q3VfBFMneM8PGsxmIGCcxpqqnLcYzpZjOzlaWi1G7nZWmeVFey~8qbwjdTPsS-rxW2PptyxjdGYiSXt1sL0AM~pj6-JqhssD0iug55RyRDBmiixxbazJbrchG7Z2bpJhpIi76426ksavERhtxmP2E8IHgLzHi~C9kX~HZDr0Tz8pGKOwhxGZv5IYD9oZqtt0ygEeeSkOmWcTpXILog__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PAI-1 in control and QPD platelets.
(A) PAI-1 in control (C) and QPD (Q; 3 patients [Pt] are shown) samples was analyzed by Western blotting with rabbit anti–human PAI-1 after 10% nonreduced SDS-PAGE. Lanes compare 5 μL lysate (L) and ionophore releasates (R), 20 μL K562 media, and 5 μL control ionophore releasate, incubated with 0 to 400 ng/mL tcu-PA (CR + tcuPA), as indicated. Lane * shows the PAI-1 affinity purified from QL using monoclonal anti–u-PA. QPD platelet lysates contained PAI-1 in high–molecular weight complexes (bands near the 98-kd marker) that comigrated with the complexes generated by adding tcu-PA to control releasates. Proteolyzed PAI-1 (arrow) was detected in QPD platelet lysates and in long exposures (not shown) of control releasates incubated with tcu-PA. (B) u-PA–PAI-1 complex ELISA indicated that, unlike control samples, QPD platelet lysates and releasates were unable to generate additional u-PA–PAI-1 complexes in vitro with added (+) tcu-PA (data representative of 2 separate experiments with pooled samples).
